Structural and biochemical investigation of heptad repeat derived peptides of human SARS corona virus (hSARS-CoV) spike protein.
Identifieur interne : 001580 ( Main/Exploration ); précédent : 001579; suivant : 001581Structural and biochemical investigation of heptad repeat derived peptides of human SARS corona virus (hSARS-CoV) spike protein.
Auteurs : Sarmistha Basak [Canada] ; Xiaolei Hao ; Andrew Chen ; Michel Chrétien ; Ajoy BasakSource :
- Protein and peptide letters [ 0929-8665 ] ; 2008.
Descripteurs français
- KwdFr :
- Analyse de séquence de protéine, Chromatographie en phase liquide à haute performance, Dichroïsme circulaire, Dénaturation des protéines, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (métabolisme), Liaison aux protéines, Peptides (), Peptides (métabolisme), Peptides (synthèse chimique), Protéines de fusion membranaire, Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (métabolisme), Spectrométrie de fluorescence, Spectrométrie de masse, Spectrométrie de masse MALDI, Structure secondaire des protéines, Structure tertiaire des protéines, Séquence d'acides aminés, Virus du SRAS (), Virus du SRAS (métabolisme), Électrophorèse sur gel de polyacrylamide.
- MESH :
- génétique : Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- métabolisme : Glycoprotéines membranaires, Peptides, Protéines de l'enveloppe virale, Virus du SRAS.
- synthèse chimique : Peptides.
- Analyse de séquence de protéine, Chromatographie en phase liquide à haute performance, Dichroïsme circulaire, Dénaturation des protéines, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Liaison aux protéines, Peptides, Protéines de fusion membranaire, Protéines de l'enveloppe virale, Spectrométrie de fluorescence, Spectrométrie de masse, Spectrométrie de masse MALDI, Structure secondaire des protéines, Structure tertiaire des protéines, Séquence d'acides aminés, Virus du SRAS, Électrophorèse sur gel de polyacrylamide.
English descriptors
- KwdEn :
- Amino Acid Sequence, Chromatography, High Pressure Liquid, Circular Dichroism, Electrophoresis, Polyacrylamide Gel, Mass Spectrometry, Membrane Fusion Proteins, Membrane Glycoproteins (chemistry), Membrane Glycoproteins (genetics), Membrane Glycoproteins (metabolism), Peptides (chemical synthesis), Peptides (chemistry), Peptides (metabolism), Protein Binding, Protein Denaturation, Protein Structure, Secondary, Protein Structure, Tertiary, SARS Virus (chemistry), SARS Virus (metabolism), Sequence Analysis, Protein, Spectrometry, Fluorescence, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism).
- MESH :
- chemical , chemical synthesis : Peptides.
- chemical , chemistry : Membrane Glycoproteins, Peptides, Viral Envelope Proteins.
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , metabolism : Membrane Glycoproteins, Peptides, Viral Envelope Proteins.
- chemical : Membrane Fusion Proteins, Spike Glycoprotein, Coronavirus.
- chemistry : SARS Virus.
- metabolism : SARS Virus.
- Amino Acid Sequence, Chromatography, High Pressure Liquid, Circular Dichroism, Electrophoresis, Polyacrylamide Gel, Mass Spectrometry, Protein Binding, Protein Denaturation, Protein Structure, Secondary, Protein Structure, Tertiary, Sequence Analysis, Protein, Spectrometry, Fluorescence, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.
Abstract
hSARS-CoV is the causative agent for SARS infection. Its spike glycoprotein (S) is processed by host furin enzyme to produce S1 and S2 fragments, the latter being crucial for fusion with the host membrane. This takes place via formation of a coiled coil 6-helix bundle involving N and C-terminal heptad repeat domains (HR-N and HR-C) of S2. Several fluorescent and non-fluorescent peptides from these domains were synthesized to examine their interactions by circular dichroism, thermal denaturation, native-page, mass spectrometry and fluorescence spectroscopy studies. Data revealed that HR-C domains (1153-1189), (1153-1172) and (1164-1184) all exhibit potent binding interactions with HR-N(892-931) domain. These peptides may find useful therapeutic applications in SARS intervention.
DOI: 10.2174/092986608785849173
PubMed: 18991761
Affiliations:
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Le document en format XML
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Desorption-Ionization</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (chemistry)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence de protéine</term><term>Chromatographie en phase liquide à haute performance</term><term>Dichroïsme circulaire</term><term>Dénaturation des protéines</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires ()</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (métabolisme)</term><term>Liaison aux protéines</term><term>Peptides ()</term><term>Peptides (métabolisme)</term><term>Peptides (synthèse chimique)</term><term>Protéines de fusion membranaire</term><term>Protéines de l'enveloppe virale ()</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Spectrométrie de fluorescence</term><term>Spectrométrie de masse</term><term>Spectrométrie de masse MALDI</term><term>Structure secondaire des protéines</term><term>Structure tertiaire des protéines</term><term>Séquence d'acides aminés</term><term>Virus du SRAS ()</term><term>Virus du SRAS (métabolisme)</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemical synthesis" xml:lang="en"><term>Peptides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Glycoproteins</term><term>Peptides</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Membrane Glycoproteins</term><term>Peptides</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Membrane Fusion Proteins</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Peptides</term><term>Protéines de l'enveloppe virale</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="synthèse chimique" xml:lang="fr"><term>Peptides</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Chromatography, High Pressure Liquid</term><term>Circular Dichroism</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Mass Spectrometry</term><term>Protein Binding</term><term>Protein Denaturation</term><term>Protein Structure, Secondary</term><term>Protein Structure, Tertiary</term><term>Sequence Analysis, Protein</term><term>Spectrometry, Fluorescence</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence de protéine</term><term>Chromatographie en phase liquide à haute performance</term><term>Dichroïsme circulaire</term><term>Dénaturation des protéines</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires</term><term>Liaison aux protéines</term><term>Peptides</term><term>Protéines de fusion membranaire</term><term>Protéines de l'enveloppe virale</term><term>Spectrométrie de fluorescence</term><term>Spectrométrie de masse</term><term>Spectrométrie de masse MALDI</term><term>Structure secondaire des protéines</term><term>Structure tertiaire des protéines</term><term>Séquence d'acides aminés</term><term>Virus du SRAS</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">hSARS-CoV is the causative agent for SARS infection. Its spike glycoprotein (S) is processed by host furin enzyme to produce S1 and S2 fragments, the latter being crucial for fusion with the host membrane. This takes place via formation of a coiled coil 6-helix bundle involving N and C-terminal heptad repeat domains (HR-N and HR-C) of S2. Several fluorescent and non-fluorescent peptides from these domains were synthesized to examine their interactions by circular dichroism, thermal denaturation, native-page, mass spectrometry and fluorescence spectroscopy studies. Data revealed that HR-C domains (1153-1189), (1153-1172) and (1164-1184) all exhibit potent binding interactions with HR-N(892-931) domain. These peptides may find useful therapeutic applications in SARS intervention.</div></front></TEI><affiliations><list><country><li>Canada</li></country></list><tree><noCountry><name sortKey="Basak, Ajoy" sort="Basak, Ajoy" uniqKey="Basak A" first="Ajoy" last="Basak">Ajoy Basak</name><name sortKey="Chen, Andrew" sort="Chen, Andrew" uniqKey="Chen A" first="Andrew" last="Chen">Andrew Chen</name><name sortKey="Chretien, Michel" sort="Chretien, Michel" uniqKey="Chretien M" first="Michel" last="Chrétien">Michel Chrétien</name><name sortKey="Hao, Xiaolei" sort="Hao, Xiaolei" uniqKey="Hao X" first="Xiaolei" last="Hao">Xiaolei Hao</name></noCountry><country name="Canada"><noRegion><name sortKey="Basak, Sarmistha" sort="Basak, Sarmistha" uniqKey="Basak S" first="Sarmistha" last="Basak">Sarmistha Basak</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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